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mouse ig staining  (Vector Laboratories)


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    Vector Laboratories mouse ig staining
    Mouse Ig Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse ig staining/product/Vector Laboratories
    Average 95 stars, based on 138 article reviews
    mouse ig staining - by Bioz Stars, 2026-03
    95/100 stars

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    Fig. 2. The acute inflammation rather than type II immune response around ECM scaffold membrane in aged wounds. (a) Schematic diagram for generating the scRNA-seq and spatial transcriptomics data from ECM mediated wound healing at POD7 in young and aged mice. (b) The UMAP reduction results reveals 13 different cell types in wounds. The marker genes are listed on the right of the annotation. (c) The annotated clustering results for spatial transcriptomic based on the anatomic structure of the wounds. (d) The proportion of each cell type of the whole wounds and their related wound healing process. (e) The upregulated results for Gene Sets Enrichment Analysis (GSEA) in ECM scaffolds treated aged wounds. (f) The AddModule scores of acute inflammation term in spatial transcriptomics expression. (g) The IF results for INOS and LY6G in Young_ECM and Aged_ECM at POD7. (Scale bars at the same column are the same) (h–i) The semiquantitative analysis of INOS and LY6G fluorescence area in (g) respectively. **P < 0.01 and *P < 0.05 by Student’s t-test (n = 5) for data in (h) and (i).

    Journal: Biomaterials

    Article Title: Integrated-omics profiling unveils the disparities of host defense to ECM scaffolds during wound healing in aged individuals.

    doi: 10.1016/j.biomaterials.2024.122685

    Figure Lengend Snippet: Fig. 2. The acute inflammation rather than type II immune response around ECM scaffold membrane in aged wounds. (a) Schematic diagram for generating the scRNA-seq and spatial transcriptomics data from ECM mediated wound healing at POD7 in young and aged mice. (b) The UMAP reduction results reveals 13 different cell types in wounds. The marker genes are listed on the right of the annotation. (c) The annotated clustering results for spatial transcriptomic based on the anatomic structure of the wounds. (d) The proportion of each cell type of the whole wounds and their related wound healing process. (e) The upregulated results for Gene Sets Enrichment Analysis (GSEA) in ECM scaffolds treated aged wounds. (f) The AddModule scores of acute inflammation term in spatial transcriptomics expression. (g) The IF results for INOS and LY6G in Young_ECM and Aged_ECM at POD7. (Scale bars at the same column are the same) (h–i) The semiquantitative analysis of INOS and LY6G fluorescence area in (g) respectively. **P < 0.01 and *P < 0.05 by Student’s t-test (n = 5) for data in (h) and (i).

    Article Snippet: For the evaluation the acute inflammatory molecules and immune cells infiltration, immunohistochemistry staining for CD3 (14-0032-82, Thermo Fisher Scientific, 1:100) and immunofluorescent staining for LY6G (65140-1-Ig, Proteintech, 1:200), INOS (22226-1-AP, Proteintech, 1:200), IL-1β (P420B, Thermo Fisher Scientific, 1:200),TNF-α (60291-1-Ig Proteintech, 1:200), OPN (22952- 1-AP, Proteintech, 1:200), F4/80 (29414-1-AP, Proteintech, 1:200), ARG1 (16001-1-AP, Proteintech, 1:150), IL-10 (ARC9102, Thermo Fisher Scientific, 1:100), CD62L (26477-1-AP Proteintech, 1:200), CD4 (67786-1-Ig, Proteintech, 1:500) were performed.

    Techniques: Membrane, Marker, Expressing, Fluorescence